ansB Antibody (HRP)

SKU: orb344901

Description

L-Asparaginase antibody (Peroxidase)

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:1,000 - 1:5,000, WB: 1:200 - 1:1,000
• Reactivity: E. coli
• Application Notes:
  Anti-Asparaginase Peroxidase conjugated antibody has been tested by western blotting to detect a single band of the expected apparent molecular weight and by ELISA. Researchers should determine optimal titers for applications that are not stated below.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Asparaginase Antibody was produced by repeated immunizations with E. coli Asparaginase protein.
• Target: ansB
• Purity: Anti-Asparaginase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Asparaginase [E. coli]. Cross reactivity against Asparaginase from other tissues and species may occur but have not been specifically determined.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-L-Asparaginase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-L-Asparaginase Antibody, L ASNase II antibody, AnsA antibody, AnsB antibody, Colaspase antibody, Cytoplasmic asparaginase I antibody, L ASNase I antibody, L asparaginase II precursor antibody, L asparagine amidohydrolase I antibody

Western Blot of Peroxidase conjugated Rabbit anti-L-ASPARAGINASE antibody. Lane 1: L-ASPARAGINASE. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase rabbit secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min RT. Predicted/Observed size: 30 kDa for L-ASPARAGINASE. Other band(s): L-ASPARAGINASE splice variants and isoforms.

Quick Database Links

Gene Symbol

ansB

UniProt

P00805

RefSeq:P00805.2