Ku70 Rabbit mAb [KD Validated]

• Catalog Number: orb1951101
• Category: Antibodies
• Description: It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together.
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: FC, ICC, IHC, IP, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human Ku70
• Antibody Type: Recombinant Antibody
• Dilution range: WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Conjugation: Unconjugated
• UniProt ID: P12956
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CTC75; CTCBF; DNA repair protein XRCC6; G22P1; Ku
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human tonsil, using Ku70 Antibody.

Western blot analysis of Ku70 expression in HeLa cell lysate.