DNA-PKcs Rabbit mAb [KD Validated]

• Catalog Number: orb1951597
• Category: Antibodies
• Description: DNA-dependent protein kinase (DNA-PK) is an important factor in the repair of double-stranded breaks in DNA. Cells lacking DNA-PK or in which DNA-PK is inhibited fail to show proper nonhomologous end-joining (NHEJ). DNA-PK is composed of two DNA-binding subunits (Ku70 and Ku86) and one 450 kDa catalytic subunit (DNA-PKcs). It is thought that a heterodimer of Ku70 and Ku86 binds to double-stranded DNA broken ends before DNA-PKcs binds and is activated.
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: ICC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human DNA-PKcs
• Antibody Type: Recombinant Antibody
• Dilution range: WB 1:1000~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Conjugation: Unconjugated
• UniProt ID: P78527
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: DNA-PKcs; DNA-dependent protein kinase catalytic s
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human breast, using DNA-PKcs Antibody.

Western blot analysis of DNA-PKcs expression in HeLa cell lysate.