KRT19 Antibody Cocktail / Cytokeratin 19

• Catalog Number: orb749644
• Category: Antibodies
• Description: Recognizes a protein of 40kDa, identified as cytokeratin-19 (CK19), which is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa and IgG2a, lambda
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant full-length human KRT19 protein was used as the immunogen for the Cytokeratin 19 antibody cocktail.
• UniProt ID: P08727
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT
• Application notes: Optimal dilution of the Cytokeratin 19 antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: KRT19/799 + KRT19/800
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Cytokeratin 19 antibody cocktail is available for research use only.
• Note: For research use only

IHC: Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Cytokeratin 19 antibody (KRT19/799 + KRT19/800).

IHC: Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Cytokeratin 19 antibody (KRT19/799 + KRT19/800).

IHC: Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Cytokeratin 19 antibody (KRT19/799 + KRT19/800).

IHC: Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with Cytokeratin 19 antibody (KRT19/799 + KRT19/800).

IHC: Formalin-fixed, paraffin-embedded human pancreas stained with Cytokeratin 19 antibody (KRT19/799 + KRT19/800).