KLF4 Antibody

• Catalog Number: orb1931518
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB17718
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: IF: 1:10~50, IF: 1:10~50, WB: 1:1000, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 54671 Da
• Target: This KLF4 antibody is generated from rabbits immunized with a his tag recombinant protein of human KLF4.
• UniProt ID: O43474
• NCBI: NP_004226.3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Krueppel-like factor 4, Epithelial zinc finger pro
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Fluorescent confocal image of HeLa cells stained with KLF4 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with KLF4 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1 h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 μM, 25 min). KLF4 immunoreactivity is localized specifically to the nuclei in HeLa cells.

Fluorescent confocal image of SY5Y cells stained with KLF4 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with KLF4 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1 h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 μM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 3 min). KLF4 immunoreactivity is localized to the nuclei in SY5Y cells.

Formalin-fixed and paraffin-embedded human colon carcinoma with KLF4 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of lysate from HT29 cell line, using KLF4 Antibody. Diluted at 1:1000. A goat anti-rabbit (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug.