KLF4 Rabbit Polyclonal Antibody

SKU: orb15894

Description

KLF4 Rabbit Polyclonal Antibody

Research Area

Cancer Research, Cardiovascular Research, Cell Biology, Cell Cycle, Cell Differentiation, Cell Markers, Cell Signaling, DNA and RNA Research, Epigenetics and Nuclear Signaling, Intercellular Signaling, Molecular Biology, Surface Molecules, Transcription Factors, Tumor Suppressors

Images & Validation

• Tested Applications: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:100-500, IF=1:50-200, Flow-Cyt=1ug/Test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Equine, Gallus, Guinea pig, Porcine, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human KLF4 (415-513/513aa)
• Target: KLF4
• Molecular Weight: 56 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Endothelial Kruppel like zinc finger protein; EZF; GKLF; KLF; Kruppel like factor 4(Epithelial zinc finger protein EZF)(Gut enriched Krueppel like factor); Kruppel like factor 4(gut); Krueppel-like factor 4; EZF; GKLF; KLF 4; KLF-4; Krueppel like factor 4; KLF4_HUMAN.

Blank control: K562. Primary Antibody (green line): Rabbit Anti-KLF4 antibody (orb15894), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: MCF7 (blue). Primary Antibody: Rabbit Anti-KLF4 AF647 Conjugated antibody, dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/AF647 (orange), used under the same conditions. Protocol, The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. The cells were washed twice with 1X PBS. The cells were incubated in 1X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (1 µg/1x10^6 cells) for 30 min on ice. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Rat lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (KLF4) Polyclonal Antibody, Unconjugated (orb15894) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: 293T (human) cell Lysate at 40 ug, Primary: Anti-KLF4 (orb15894) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kD, Observed band size: 60 kD.

Sample: Hela Cell (Human) Lysate at 30 ug, Primary: Anti-KLF4 (orb15894) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56kD, Observed band size: 56kD.

Sample: Lane 1: Mouse NIH/3T3 cell lysates, Lane 2: Human A431 cell lysates, Lane 3: Human LOVO cell lysates, Lane 4: Human HeLa cell lysates, Lane 5: Human MCF-7 cell lysates, Lane 6: Human A549 cell lysates, Primary: Anti-KLF4 (orb15894) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kDa, Observed band size: 50 kDa.

Quick Database Links

Gene Symbol

KLF4

UniProt

O43474