KLF4 Antibody

• Catalog Number: orb1931517
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB18934
• Tested applications: IF, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: IF: 1:25, IF: 1:10~50, IF: 1:10~50, WB: 1:1000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 54671 Da
• Target: This KLF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 481-504 amino acids from human KLF4.
• UniProt ID: O43474
• NCBI: NP_004226.3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Krueppel-like factor 4, Epithelial zinc finger pro
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent analysis of U251 cells, using KLF4 Antibody. Diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).

Fluorescent confocal image of HeLa cells stained with KLF4 (S457) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with KLF4 (S457) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1 h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 μM, 25 min).

Fluorescent confocal image of SY5Y cells stained with KLF4 (S457) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with KLF4 (S457) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1 h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 μM, 25 min).

Western blot analysis of KLF4-S457 in MDA-MB231, NCI-H460 cell line lysates (35 ug/lane). KLF4 (arrow) was detected using the purified Pab.