KIF5B Antibody

SKU: orb750523

Description

KIF5B antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:10000, WB: 1:100-1:500
• Reactivity: Human
• Application Notes:
  Kinesin-1 antibody has been tested for use in SDS, western blot, and immunohistochemistry. For western blots expect a band of approximately 72 kDa in size corresponding to truncated kinesin-1 protein. Specific conditions for reactivity should be optimized by the end user.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Antiserum
• Immunogen: Anti-Kinesin-1 was prepared from whole rabbit serum produced by repeated immunizations with a truncated kinesin-1 construct expressed in E. coli corresponding to human kinsesin-1protein.
• Target: KIF5B
• Purity: Anti-Kinesin-1 is directed against the human kinesin-1 protein. The product was prepared from monospecific antiserum by delipidation and defibrination. A BLAST analysis was used to suggest reactivity with human. Cross-reactivity with kinesin 1 from other sources have not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 77 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Kinesin 1 Antibody, Conventional kinesin heavy chain, Ubiquitous kinesin heavy chain, KNS, KNS1, Kinesin-1 heavy chain, UKHC, Anti-Kinesin-1 antibody, kinesin 1 antibody, kin1, kin-1, KIF5, KHC, Kinesin motor domain

Immunohistochemistry of Rabbit anti-Kinesin-1. Tissue: Human Brain at 40X at pH6.

SDS-PAGE of Truncated Kinesin 1 Protein. Lane 1: Truncated Kinesin-1 Protein. Lane 2: none. Load: 5 ug per lane. Block: coomassie blue for 20 min, destain overnight at RT. Predicted/Observed size: 72 kDa, 72kDa for kinesin-1. Other band(s): degradation.

Western Blot of Rabbit anti-Kinesin 1 Antibody. Lane 1: 500 ng of truncated kinesin 1 protein. Lane 2: 20 ug of Mouse Brain lysate. Lane 3: 10 ug of Mouse Brain lysate. Primary antibody: kinesin1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:40000 for 1hr at RT. Block: 5% BLOTTO 1 hr at RT. Predicted/Observed size: 72 kDa, ~70 kDa for kinesin-1. Other band(s): degradation.

Quick Database Links

Gene Symbol

KIF5B

UniProt

P33176

RefSeq (Protein):NP_004512.1