KIF4A Antibody

SKU: orb1247341

Description

KIF4A Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Porcine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 150kDa band observed in nuclear lysates of cell line NIH3T3 (calculated MW of 139.5kDa according to Mouse NP_032472.2). Recommended concentration:1-2µg/mL. Primary incubation 1 hour at room temperature. Negative Control: Human Ovary lysate.Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10µg/mL. Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10µg/mL.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: SFFSGCSPIEEEAH
• Target: KIF4A
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

KIF4A, kinesin family member 4A, FLJ12530, FLJ12655, FLJ14204, FLJ20631, HSA271784, KIF4, KIF4-G1, chromokinesin, kinesin family member 4

Quick Database Links

Gene Symbol

KIF4A

UniProt

O95239

RefSeq (Protein):NP_036442.3