KIF4A Antibody

SKU: orb18420

Description

Goat polyclonal antibody to KIF4A

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution Range: ELISA: 1:64000, WB: 1-2 μg/ml, IHC-P: 10ug/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments in lysates of cell line HeLa gave no specific signal but low background (at antibody concentration up to 1 μg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysatesExperiment Notes: Immunofluorescence: This antibody has been successfully used in ICC/IF on HeLa (methanol-fixed, and BSA-blocked) by a customer.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: KIF4A
• Protein Sequence: SFFSGCSPIEEEAH
• Molecular Weight: 140
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti KIF4A antibody, anti kinesin family member 4A antibody, anti FLJ12530 antibody, anti FLJ12655 antibody, anti FLJ14204 antibody, anti FLJ20631 antibody, anti HSA271784 antibody, anti KIF4 antibody, anti KIF4-G1 antibody, anti chromokinesin antibody, anti kinesin family member 4 antibody

Flow cytometric analysis of HEK293 cells using KIF4A antibody

Western blot analysis of NIH3T3(lane 1) and Human Ovary(lane 2) lysate using KIF4A antibody

Immunofluorescence analysis of U2OS cells using KIF4A antibody

Quick Database Links

Gene Symbol

KIF4A