Ki-67 Antibody

• Catalog Number: orb1151559
• Category: Antibodies
• Description: Mouse monoclonal antibody to Ki-67
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A portion of amino acids 2293-2478 was used as the immunogen for the recombinant Ki67 antibody.
• UniProt ID: P46013
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the recombinant Ki67 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: rMKI67/6615
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Epigenetics
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human lymph node with recombinant Ki67 antibody (clone rMKI67/6615). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with recombinant Ki67 antibody (clone rMKI67/6615). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC staining of FFPE human brain tissue with recombinant Ki67 antibody (clone rMKI67/6615) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant Ki67 antibody (clone rMKI67/6615) as confirmation of integrity and purity.