Rabbit anti-Ki-67 Recombinant Monoclonal Antibody

SKU: orb1519949

Description

Ki-67 Antibody

Images & Validation

• Tested Applications: FC, ICC, IF, IHC
• Dilution range: Flow-Cyt Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells. Immunocytochemistry (ICC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. ICC-IF 1:100 - 1:500. Immunohistochemistry (IHC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. IHC-IF 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Multiplex Immunofluorescence (mIF) 1:250
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Clone No.: BLR021E
• Immunogen: Between 1200 and 1250
• Target: Ki-67
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 50 µg/ml
• Disclaimer: For research use only

Alternative Names

PPP1R105; antigen identified by monoclonal antibody Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; proliferation marker protein Ki-67; MIB-1; MIB-; KIA; antigen Ki67; Antigen KI-67

Detection of human Ki-67 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-Ki-67 recombinant monoclonal antibody (orb1519949). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human Ki-67 (shaded) in MOLT4 cells by flow cytometry. Antibody: Rabbit anti-Ki-67 recombinant monoclonal (orb1519949) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human Ki-67 by immunocytochemistry. Sample: FFPE section of MOLT-4 cells. Antibody: Rabbit anti-Ki-67 recombinant monoclonal antibody (orb1519949). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human Ki-67 by immunocytochemistry. Sample: acetone/methanol fixed Hela cells. Antibody: Rabbit anti-Ki-67 recombinant monoclonal antibody (orb1519949) used at 1:100. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG. Counterstain: DAPI.

Detection of human Ki-67 (red) by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-Ki-67 recombinant monoclonal antibody (orb1519949) used at 1:500. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).

Quick Database Links

Gene Symbol

Ki-67

UniProt

P46013

RefSeq (Protein):NP_002408.3