Rabbit anti-Ki-67 Recombinant Monoclonal Antibody

• Catalog Number: orb1519949
• Category: Antibodies
• Description: Ki-67 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BLR021E
• Tested applications: FC, ICC, IF, IHC
• Reactivity: Human
• Immunogen: Between 1200 and 1250
• Antibody Type: Primary Antibody
• Concentration: 50 µg/ml
• Dilution range: Flow-Cyt Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells. Immunocytochemistry (ICC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. ICC-IF 1:100 - 1:500. Immunohistochemistry (IHC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. IHC-IF 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Multiplex Immunofluorescence (mIF) 1:250
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Ki-67
• UniProt ID: P46013
• NCBI: NP_002408.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: PPP1R105; antigen identified by monoclonal antibod
• Background: Ki-67 is a marker of cell proliferation detected in cells that are in G1-, S-, G2-, and M-phase of the cell cycle and absent in cells in G0. Ki-67 is detected exclusively in the nucleus, and its detection by immunohistological methods has proven to have diagnostic and prognostic value in the study of a variety of human tumors. The specific cellular functions of Ki-67 have not been elucidated, but it is proposed to function in the organization and maintenance of the architecture of DNA and in the synthesis of ribosomes during mitosis
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Ki-67 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-Ki-67 recombinant monoclonal antibody (orb1519949). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human Ki-67 (shaded) in MOLT4 cells by flow cytometry. Antibody: Rabbit anti-Ki-67 recombinant monoclonal (orb1519949) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human Ki-67 by immunocytochemistry. Sample: FFPE section of MOLT-4 cells. Antibody: Rabbit anti-Ki-67 recombinant monoclonal antibody (orb1519949). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human Ki-67 by immunocytochemistry. Sample: acetone/methanol fixed Hela cells. Antibody: Rabbit anti-Ki-67 recombinant monoclonal antibody (orb1519949) used at 1:100. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG. Counterstain: DAPI.

Detection of human Ki-67 (red) by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-Ki-67 recombinant monoclonal antibody (orb1519949) used at 1:500. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).