KDEL Antibody: FITC

• Catalog Number: orb413471
• Category: Antibodies
• Description: Mouse monoclonal antibody against KDEL conjugated to FITC
• Target: KDEL
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: FITC
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: 640.91mM DMSO, 136.36mM Ethanolamine, 9.09mM Sodium Bicarbonate in 90.9% PBS
• Immunogen: KDEL peptide (C-AVKDEL -COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA)
• Tested applications: ICC, IF, IHC
• Dilution range: WB (1:1000); IHC (1:25)
• Application notes: A 1:1000 dilution of SMC-539 was sufficient for detection of KDEL in 10 µg of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Clone Number: 2C1
• Storage: Conjugated antibodies should be stored according to the product label
• Alternative names: KDEL antibody, Lys Asp Glu Leu antibody, Lys-Asp-G
• Note: For research use only

Immunohistochemistry analysis using Mouse Anti-KDEL Monoclonal Antibody, Clone 2C1. Tissue: kidney. Species: Rat. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) KDEL Antibody (D) Composite.

Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 2C1. Lane 1: Molecular Weight Ladder (MW). Lane 2: Anti-KDEL. Lane 3: Anti-KDEL control. Lane 4: Anti-PDI control. Lane 5: Anti-Calreticulin control. Lane 6: Anti-GRP78 control. Lane 7: Anti-GRP94 control. Load: 10 μg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.