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| Catalog Number | orb1247345 |
|---|---|
| Category | Antibodies |
| Description | KCC1 Antibody |
| Target | SLC12A4 |
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Predicted Reactivity | Canine, Mouse, Rat |
| Form/Appearance | Liquid |
| Concentration | 500 ug/mL |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Immunogen | The immunogen for this antibody is: C-DPSHAPDNFRE |
| UniProt ID | Q9UP95 |
| Tested applications | ELISA, IHC, WB |
| Application notes | Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 150kDa band observed in Human Heart lysates (calculated MW of 121kDa according to NP_005063.1). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:Paraffin embedded Human Placenta. Recommended concentration: 5ug/ml. |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | SLC12A4, KCC1, solute carrier family 12 (potassium Read more... |
| Research Area | Cancer Biology |
| Note | For research use only |
| NCBI | NP_005063.1 |

orb1247345 (0.3 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247345 (5 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Rat | |
0.32-20 ng/mL | |
0.125 ng/mL |
Human | |
0.16-10 ng/mL | |
0.048 ng/mL |
ELISA, IHC, WB | |
Canine, Mouse, Rat | |
Human | |
Goat | |
Polyclonal | |
Unconjugated |
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