KCC1/SLC12A4 Antibody

• Catalog Number: orb19634
• Category: Antibodies
• Description: Goat polyclonal antibody to SLC12A4
• Target: KCC1 / SLC12A4
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: DPSHAPDNFRE
• RRID: AB_10750964
• MW: 121
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:4000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 150kDa band observed in Human Heart and Skeletal Muscle lysates (calculated MW of 121kDa according to NP_005063.1). Recommended concentration: 0.3-1 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti SLC12A4 antibody, anti KCC1 antibody, anti so
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 6560

orb19634 (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19634 (5µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.