IWP-2

SKU: orb1305145

Description

IWP-2

Research Area

Metabolism Research, Signal Transduction, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 686770-61-6
• MW: 466.6
• Purity: 99.76%
• Formula: C₂₂H₁₈N₄O₂S₃
• SMILES: Cc1ccc2nc(NC(=O)CSc3nc4CCSc4c(=O)n3-c3ccccc3)sc2c1
• Target: Porcupine,Wnt/beta-catenin,Casein Kinase
• Solubility: DMSO:1 mg/mL (2.14 mM)

Bioactivity

• Target IC50:
  Wnt:27 nM|CK1δ:40 nM
• In Vivo:
  METHODS: To study the effects on hair loss, C57/BL6 mice were treated with AA antioxidant (100 mg/mL, topically applied to the skin), PBMT, and IWP-2 (20 mg/kg, i.v., every two days). RESULTS: PBMT promoted hair regrowth after resting shaving stimulation. Importantly, the use of AA and IWP-2 eliminated the differences in HF growth rates induced by PBMT in vivo.
• In Vitro:
  METHODS: Human gastric cancer cells MKN28 were treated with IWP-2 (5-50 µM) for 1-5 days, and cell proliferation was detected by CellTiter Assay. RESULTS: After four days of treatment in MKN28 cell line, 10-50 µM IWP-2 significantly inhibited the proliferation of MKN28 cells. METHODS: L-Wnt3A cells were treated with IWP-2 (5 µM) for 24 h, and the expression levels of target proteins were detected by Western Blot. RESULTS: IWP-2 blocked all detected Wnt-dependent biochemical changes, phosphorylation of Lrp6 receptor and Dvl2, and accumulation of β-catenin.
• Cell Research:
  IWP-2 is prepared in DMSO and stored, and then diluted with appropriate medium before use. The human RCC cell lines 786O and Caki-2 (5×103) are seeded into 96-well plates. Cell viability is estimated by MST assay after Caki-2 acells are incubated with ncreasing concentrations of LEF together with 20 μM IWP-2 for 48 h.After treatment, 10 μL MTS is added into each well for 2 h incubation. The absorbance is measured using a model ELX800 Micro Plate Reader at 490 nm. For colony formation assay, Caki-2 cells are trypsinized to single cell suspensions and seeded into fresh 6-well plates at 1000 cells/well. Then cells are incubated with LEF at depicted concentrations for 7 days. Colonies are fixed with absolute methanol for 15 min and then stained with 0.1% crystal violet for 20 min. After washing with PBS three times, the colonies with a diameter over 2 mm are visualized by a digital camera.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

beta-catenin, betacatenin, CaseinKinase, Casein Kinase, bcatenin, Inhibitor, inhibit, IWP2, IWP-2, IWP 2, Wnt/β-catenin, Wnt/betacatenin, Wnt, Wnt/b-catenin, Porcn, Porcupine, βcatenin

Compound Identifiers

PubChem CID

2155128

Qingnian Liu 1, Jiniu Huang 1, Hao Ding 1, Yue Tao 1, Jinliang Nan 1, Changchen Xiao 1, Yingchao Wang 1, Rongrong Wu 1, Cheng Ni 1, Zhiwei Zhong 1, Wei Zhu 2, Jinghai Chen 3, Chenyun Zhang 1, Xiao He 4, Danyang Xiong 4, Xinyang Hu 1, Jian'an Wang Flavin-containing monooxygenase 2 confers cardioprotection in ischemia models through its disulfide-bond catalytic activity J Clin Invest, (2024)

PubMed: 39480513