IWP-2

SKU: orb1224243

Description

IWP-2 is an inhibitor of Wnt processing and secretion with IC50 of 27 nM in a cell-free assay, selective blockage of Porcn-mediated Wnt palmitoylation, does not affect Wnt/β-catenin in general and displays no effect against Wnt-stimulated cellular responses.

Key Properties

• CAS Number: 686770-61-6
• MW: 466.6
• Purity: >98% (HPLC)
• Formula: C₂₂H₁₈N₄O₂S₃
• SMILES: O=C(NC1=NC2=CC=C(C)C=C2S1)CSC3=NC(CCS4)=C4C(N3C5=CC=CC=C5)=O
• Target: Wnt/beta/catenin
• Solubility: DMF: 22 mg/mL warming (47.14 mM); DMSO: 4 mg/mL warming (8.57 mM)

Bioactivity

• In Vitro:
  IWP-2 (GMP) (2 μM, in the first 4 days of Stage IV induction medium) reprograms human somatic cells to pluripotent stem cells. IWP-2 (GMP) (5 μM, day 3 to 5) induces cardiac differentiation of hiPSCs. IWP-2 (GMP) (5 μM, day 1 to 3) increasing the expression of cardiac progenitors and cardiac genes (MYL2, TNNI3, and TNNT2) in hiPSCs. IWP-2 (GMP) (5 μM, day 5-7) together with PIP-S2 induces cardiac mesoderm differentiates into functional cardiomyocytes. IWP-2 (GMP) (5 μM, treated at day 3) induces cardiomyocyte differentiation when applied following a pretreatment with Laduviglusib (GMP) (HY-10182G). RT-PCR Cell line: hiPSCs. Concentration: 5 μM. Incubation time: day 1 to 3. Result: Reduced the expression of anti-cardiac mesoderm genes, and increased the expression of cardiac progenitors and cardiac genes (MYL2, TNNI3, and TNNT2).
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor of Wnt Production-2

Quick Database Links

Gene Symbol

Wnt/beta/catenin