ITGAL Antibody

• Catalog Number: orb1248939
• Category: Antibodies
• Description: ITGAL Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-YQVRIQPSIHDHN
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 200kDa band observed in Human Spleen and Tonsil lysates (calculated MW of 129kDa according to NP_002200.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-2ug/ml.Immunohistochemistry:In paraffin embedded Human Lymph Node shows membrane staining in the paracortex. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Spleen. Recommended concentration: 3.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ITGAL
• UniProt ID: P20701
• NCBI: NP_002200.2, NP_001107852.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: ITGAL, integrin, alpha L (antigen CD11A (p180), ly
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 200kDa band observed in Human Spleen and Tonsil lysates (calculated MW of 129kDa according to NP_002200.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-2ug/ml.Immunohistochemistry:In paraffin embedded Human Lymph Node shows membrane staining in the paracortex. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Spleen. Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248939 (0.5 ug/ml) staining of Human Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248939 (2 ug/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

orb1248939 Negative Control showing staining of paraffin embedded Human Lymph Node, with no primary antibody.

orb1248939 (3.75 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.