Goat anti-CD11A / integrin alpha L Antibody

SKU: orb20269

Description

Goat polyclonal antibody to ITGAL

Images & Validation

• Tested Applications: ELISA, FC, IHC, WB
• Dilution range: ELISA: 1:16000, WB: 0.5-2 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 200kDa band observed in Human Spleen and Tonsil lysates (calculated MW of 129kDa according to NP_002200.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-2 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: CD11A / integrin alpha L
• Molecular Weight: 129; 119
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti ITGAL antibody, anti integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1 antibody, anti alpha polypeptide) antibody, anti CD11A antibody, anti LFA-1 antibody, anti LFA1A antibody, anti LFA-1 alpha antibody, anti antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide antibody, anti integrin alpha L antibody, anti integrin gene promoter antibody, anti lymphocyte function-associated antigen 1 antibody

3 μg/ml staining of THP-1 cell lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.

2 μg/ml staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

3.75 μg/ml staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Flow cytometric analysis of paraformaldehyde fixed THP1 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Quick Database Links

Gene Symbol

CD11A / integrin alpha L

NCBI Gene ID

3683