Goat anti-CD11A / integrin alpha L Antibody

• Catalog Number: orb20269
• Category: Antibodies
• Description: Goat polyclonal antibody to ITGAL
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IHC, WB
• Reactivity: Human
• Dilution range: ELISA: 1:16000, WB: 0.5-2 μg/ml, IHC-P: 3.75 μg/ml
• Conjugation: Unconjugated
• MW: 129; 119
• Target: CD11A / integrin alpha L
• Entrez: 3683
• Protein Sequence: YQVRIQPSIHDHN
• RRID: AB_10752816
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti ITGAL antibody, anti integrin, alpha L (antig
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 200kDa band observed in Human Spleen and Tonsil lysates (calculated MW of 129kDa according to NP_002200.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-2 μg/ml.
• Expiration Date: 12 months from date of receipt.

3 μg/ml staining of THP-1 cell lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.

2 μg/ml staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

3.75 μg/ml staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Flow cytometric analysis of paraformaldehyde fixed THP1 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.