ITGA4/VLA-4/CD49d Antibody

SKU: orb1538168

Description

ITGA4/VLA-4/CD49d Antibody

Images & Validation

• Tested Applications: ELISA, IF, IHC, IHC-P, WB
• Dilution Range: ELISA, IF, IHC, IHC-P (5 µg/ml), WB (0.5 - 1 µg/ml)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Further information: Integrin alpha 4 antibody can be used for the detection of Integrin alpha 4 by Western blot at 1 ug/mL. Despite its predicted molecular weight, Integrin alpha4 will often run at higher molecular weight in SDS-PAGE. Antibody can also be used for immunoflourescence starting at 2 ug/mL. Antibody validated: Western Blot in rat samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot:

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A 15 AA synthetic peptide near the center of human Integrin alpha 4. The immunogen is located within aa 220-270 of Integrin alpha 4.
• Target: ITGA4 / VLA-4 / CD49d
• Purification: Peptide Column Affinity Chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS containing 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ITGA4, 269C wild type, CD49D, IA4, Integrin alpha-4, Integrin alpha 4, Integrin alpha-4 subunit, Integrin alpha-IV, VLA-4, VLA-4 subunit alpha, CD49d antigen

Immunofluorescence of Integrin alpha 4 in Jurkat cells with Integrin alpha 4 antibody at 2 ug/ml.

Western blot of Integrin alpha 4 in rat spleen tissue lysate with Integrin alpha 4 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.

Quick Database Links

Gene Symbol

ITGA4 / VLA-4 / CD49d