ITGA4/VLA-4/CD49d Antibody

SKU: orb1534825

Description

ITGA4/VLA-4/CD49d Antibody

Images & Validation

• Tested Applications: IHC, IHC-P
• Dilution Range: IHC, IHC-P (10 - 20 µg/ml)
• Reactivity: Human, Monkey, Mouse, Rat
• Application Notes:
  Further information: Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 10 - 20 ug/ml.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic 14 amino acid peptide from internal region of human ITGA4 / VLA-4. Percent identity with other species by BLAST analysis: Human (100%), Gorilla (100%), Gibbon (100%), Monkey (100%), Marmoset (100%), Mouse (100%), Rat (100%), Elephant (93%), Panda (93%), Dog (86%), Bat (86%), Bovine (86%), Rabbit (86%), Horse (86%), Pig (86%).
• Target: ITGA4 / VLA-4 / CD49d
• Purification: Immunoaffinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ITGA4, 269C wild type, CD49D, IA4, Integrin alpha-4, Integrin alpha 4, Integrin alpha-4 subunit, Integrin alpha-IV, VLA-4, VLA-4 subunit alpha, CD49d antigen

Anti-VLA-4 / CD49D antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10-20 ug/ml.

Quick Database Links

Gene Symbol

ITGA4 / VLA-4 / CD49d