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ISG15 Antibody

Catalog Number: orb344617

DispatchUsually dispatched within 5-10 working days
$ 800.00
Catalog Numberorb344617
CategoryAntibodies
DescriptionISG15 antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, WB
ReactivityHuman
IsotypeIgG
ImmunogenThis purified antibody was prepared from rabbit serum after repeated immunizations with recombinant human ISG15 protein.
Antibody TypePrimary Antibody
Concentration5.0 mg/mL
Dilution rangeELISA: 1:2,000 - 1:10,000, WB: 1:200 - 1:1,000
Form/AppearanceLyophilized
PurityThis product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
ConjugationUnconjugated
UniProt IDP05161
NCBI4826774
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Buffer/Preservatives0.01% (w/v) Sodium Azide
Alternative namesrabbit anti-ISG15 Antibody, G1P2 antibody, IFI 15
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NoteFor research use only
Application notesThis purified polyclonal antibody reacts with human ISG15 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or conjugates), especially at lower dilutions. An 18.5 kDa band corresponding to human ISG15 is detected. IFNα or IFNß stimulated HeLa cell lysates can be used as a positive control.
Expiration Date12 months from date of receipt.
ISG15 Antibody

Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF (SKP1/cullin-1/F-box protein) / CBC (cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin.

ISG15 Antibody

Western blot validation of host protein regulation. A, HeLa cells were mock-infected or infected for 24 h, or B, for indicated periods of time, harvested and lysed with 0.5% NP-40 detergent. The cytosolic and nuclear fractions were separately purified, dissolved in SDS electrophoresis sample buffer, and proteins resolved in 10% (A), or in 4-16% gradient (B) SDS-PAGE, transferred to PVDF, and probed with indicated antibodies. Antibody binding was detected with HRP-conjugated secondary antibodies and ECL, and visualized with an Alpha Innotech FluorChemQ MultiImage III instrument. Molecular weight standards are indicated at left and SILAC-measured ratios are indicated on right in A. *: not detected in indicated fraction; †: based on single peptide only.

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