IRF6 Antibody

• Catalog Number: orb1247355
• Category: Antibodies
• Description: IRF6 Antibody
• Target: IRF6
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-TPSMQLPPALPPQ
• UniProt ID: O14896
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa band observed in Human Ovary lysates (calculated MW of 53.1kDa according to NP_006138.1). In transfected HEK293 transiently expressing IRF6 a band of approx. 55kDa is observed. This band is not observed in the non-transfected HEK293. Recommended for use at 0.2-0.6ug/ml.Immunohistochemistry:In paraffin embedded Human Kidney shows strong nuclear staining in DCT and glomerulus. Recommended concentration: 2-3ug/ml. Paraffin embedded Human Prostate. Recommended concentration: 5ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IRF6, interferon regulatory factor 6, LPS, PIT, PP
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_006138.1

orb1247355 staining (0.2 ug/ml) of Human Ovary lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

HEK293 overexpressing IRF6 and probed with orb1247355 (mock transfection in first lane).

orb1247355 (2.5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247355 (5 ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.