Goat anti-IRF6 Antibody

SKU: orb18656

Description

Goat polyclonal antibody to IRF6

Research Area

Epigenetics, Stem Cells

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 0.2-0.6 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Kidney shows strong nuclear staining in DCT and glomerulus. Recommended concentration, 2-3ug/ml.WB: Approx 60kDa band observed in Human Ovary lysates (calculated MW of 53.1kDa according to NP_006138.1). In transfected HEK293 transiently expressing IRF6 a band of approx. 55kDa is observed. This band is not observed in the non-transfected HEK293. Recommended for use at 0.2-0.6 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: IRF6
• Protein Sequence: TPSMQLPPALPPQ
• Molecular Weight: 53.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti IRF6 antibody, anti interferon regulatory factor 6 antibody, anti LPS antibody, anti PIT antibody, anti PPS antibody, anti VWS antibody, anti OFC6 antibody

HEK293 overexpressing IRF6 (RC201579) and probed with orb18656 (mock transfection in first lane), tested by Origene.

orb18656 (2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western blot analysis of Human Ovary lysate using IRF6 antibody

Immunohistochemical staining of Human Prostate using IRF6 antibody

Quick Database Links

Gene Symbol

IRF6

NCBI Gene ID

3664