IRAK4 Antibody

SKU: orb1248485

Description

IRAK4 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, FC, ICC, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Mouse
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa band observed in lysates of cell line HeLa and approx 55kDa in lysates of cell line Jurkat (calculated MW of 51.5kDa according to NP_057207.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.5-2µg/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Heart and Human Liver. Recommended concentration: 3.75µg/ml. Immunofluorescence: Strong expression of the protein seen in the cytoplasm and microtubules of HeLa cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: NKPITPSTYVRC
• Target: IRAK4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IRAK4, REN64, NY-REN-64, interleukin-1 receptor-associated kinase 4, IRAK-4 mutated form 1, interleukin-1 receptor associated kinase 4, interleukin-1 receptor associated kinase 4 mutant form 1, IPD1

orb1248485 (1 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248485 (3.75 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1248485 (1 μg/ml) staining of HeLa (A) and Jurkat (B) cell lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.

Quick Database Links

Gene Symbol

IRAK4

UniProt

Q9NWZ3

RefSeq (Protein):NP_001107654.1, NP_057207.2