Irak2 Antibody

SKU: orb1238891

Description

Irak2 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, ICC, IF, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Rat
• Application Notes:
  IRAK2 antibody can be used for detection of IRAK2 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: IRAK2 antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of mouse IRAK2a.The immunogen is located within the last 50 amino acids of IRAK2.
• Target: Irak2
• Molecular Weight: Predicted: 68 kDa Observed: 65 kDa
• Purification: IRAK2 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: IRAK2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IRAK2 Antibody: IRAK-2, AI649099, 6330415L08Rik, Interleukin-1 receptor-associated kinase-like 2, IRAK-2

Western blot analysis of IRAK2 in RAW264.7 whole cell lysate with mIRAK2 antibody at (A) 0.5, (B) 1, and (C) 2 µg/mL.

Immunocytochemistry of IRAK2 in A-20 cells with IRAK2 antibody at 1 µg/mL.

Immunofluorescence of IRAK2 in A20 cells with IRAK2 antibody at 10 µg/mL.

Immunofluorescence of IRAK-2 in Hela cells with IRAK 2 antibody at 20 µg/mL. Red: IRAK2 Antibody (orb1238891) Blue: DAPI staining.

Quick Database Links

Gene Symbol

Irak2

UniProt

Q8CFA1

RefSeq:AAO24761