Irak2 Antibody

• Catalog Number: orb1238891
• Category: Antibodies
• Description: Irak2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, WB
• Predicted Reactivity: Bovine, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: IRAK2 antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of mouse IRAK2a.The immunogen is located within the last 50 amino acids of IRAK2.
• Concentration: 1 mg/mL
• Dilution range: IRAK2 antibody can be used for detection of IRAK2 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 68 kDa Observed: 65 kDa
• Target: Irak2
• UniProt ID: Q8CFA1
• NCBI: AAO24761
• Storage: IRAK2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: IRAK2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: IRAK2 Antibody: IRAK-2, AI649099, 6330415L08Rik, I
• Note: For research use only
• Application notes: IRAK2 antibody can be used for detection of IRAK2 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of IRAK2 in RAW264.7 whole cell lysate with mIRAK2 antibody at (A) 0.5, (B) 1, and (C) 2 µg/mL.

Immunocytochemistry of IRAK2 in A-20 cells with IRAK2 antibody at 1 µg/mL.

Immunofluorescence of IRAK2 in A20 cells with IRAK2 antibody at 10 µg/mL.

Immunofluorescence of IRAK-2 in Hela cells with IRAK 2 antibody at 20 µg/mL. Red: IRAK2 Antibody (orb1238891) Blue: DAPI staining.