IRAK2 Antibody

SKU: orb1238892

Description

IRAK2 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, ICC, IF, WB
• Reactivity: Human
• Predicted Reactivity: Mouse
• Application Notes:
  IRAK-2 antibody can be used for detection of IRAK2 by Western blot at 2 μg/mL. A 65 kDa band should be detected. This polyclonal antibody can also detect IRAK2 by immunocytochemistry at 2 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: IRAK2 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human IRAK2.The immunogen is located within amino acids 530 - 580 of IRAK-2.
• Target: IRAK2
• Molecular Weight: 65 kDa
• Purification: IRAK-2 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: IRAK-2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IRAK-2 Antibody: IRAK-2, Interleukin-1 receptor-associated kinase-like 2, IRAK-2

Western blot analysis of IRAK2 in HeLa (H) and K562 (K) whole cell lysate with IRAK2 antibody (C2) at 2 µg/mL.

Immunocytochemical staining of HeLa cells using IRAK2 antibody at 2 µg/mL.

Immunofluorescence of IRAK-2 in Hela cells with IRAK-2 antibody at 10 µg/mL.

Immunofluorescence of IRAK-M in human spleen tissue with IRAK M antibody at 20 µg/mL. Green: IRAK-2 Antibody (orb1238892) Blue: DAPI staining.

Quick Database Links

Gene Symbol

IRAK2

UniProt

O43187

RefSeq:AF026273