IL6 Human Monoclonal Antibody

SKU: orb2960425

Description

InVivoMAb Anti-Human IL6 (BKX2244) is a human monoclonal antibody detecting Hybridoma growth factor in ELISA, FCM, Neutralization. Suitable for Human. Highlights Multi-Application: Validated across multiple applications. Flow Cytometry: Validated for flow cytometry applications.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, FC, NeA
• Reactivity: Human

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: BKX2244
• Purity: >95% as determined by SDS-PAGE.
• Purification: Protein A/G purified from cell culture supernatant.
• Endotoxin: Please contact with the lab for this information.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS, pH 7.4.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Hybridoma growth factor, BSF-2, IFN-beta-2, CDF, IL6, Interleukin-6, IL-6, B-cell stimulatory factor 2, Interferon beta-2, IFNB2, CTL differentiation factor

Flow-cytometry using anti-human IL6 antibody. IL6 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL6 antibody monoclonal antibody (Catalog # orb2960425, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human IL6 antibody. LPS treated THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL6 antibody monoclonal antibody (Catalog # orb2960425, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for InVivoMAb Anti-Human IL6.

Western blot analysis was performed using anti-IL6 monoclonal antibody at 1 µg/mL on various samples. Lane 1: recombinant human IL6 (Catalog No: orb2969496), 50 ng, Lane 2: recombinant human IL6 (Catalog No: orb2969496), 30 ng, Lane 3: recombinant human IL6 (Catalog No: orb2969496), 20 ng, Lane 4: recombinant human IL6 (Catalog No: orb2969496), 10 ng.

Quick Database Links

UniProt

P05231