Human IL-6 Mouse Monoclonal Antibody

SKU: orb499747

Description

Human IL-6 Mouse Monoclonal Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:2000-20000, IHC-P=1:200-800, IHC-F=1:200-800, IF=1:200-800
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: B3E6
• Immunogen: Recombinant human IL-6 protein 30-212 aa (C-6x His-Tag)
• Target: IL6
• Molecular Weight: 23 kDa
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid or Lyophilized
• Buffer/Preservatives: Liquid form: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. Lyophilized form: PBS only.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Il-6; ILg6; Ifnb2; IL6_MOUSE; Il6; B-cell hybridoma growth factor; Interleukin HP-1; IL6_RAT; BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IL6_HUMAN; B-cell stimulatory factor 2 (BSF-2); CTL differentiation factor (CDF); Hybridoma growth factor; Interferon beta-2 (IFN-beta-2); interleukin 6; interleukin 6 (interferon, beta 2); interferon, beta 2

Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer. Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min. Antibody incubation with Human IL-6 Mouse Monoclonal Antibody (orb499747) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

HUVEC (H) cells were treated with or without LPS (0.5 µg/ml, 24h) and Brefeldin A (300 ng/ml, 20h), 25 µg total protein per lane of cell lysates probed with human IL-6 monoclonal antibody, unconjugated (orb499747) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Quick Database Links

Gene Symbol

IL6

UniProt

P05231