Human IL-6 Mouse Monoclonal Antibody

SKU: orb499747

Description

Human IL-6 Mouse Monoclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:2000-20000, IHC-P=1:200-800, IHC-F=1:200-800, IF=1:200-800
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: 1C4
• Immunogen: Recombinant human IL-6 protein (30-212/212aa (C-6x His-Tag)aa)
• Target: IL6
• Molecular Weight: 23 kDa
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Interleukin BSF 2; B cell differentiation factor; B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; Cytotoxic T cell differentiation factor; Hepatocyte stimulating factor; HGF; HPGF; HSF; Hybridoma growth factor; Hybridoma plasmacytoma growth factor; IFNB2; IL 6; IL6 protein; Interferon beta 2; Interleukin 6(interferon beta 2); Interleukin 6; Interleukin-6; Interleukin6; IL6_HUMAN.

HUVEC (H) cells were treated with or without LPS (0.5 µg/ml, 24h) and Brefeldin A (300 ng/ml, 20h), 25 µg total protein per lane of cell lysates probed with human IL-6 monoclonal antibody, unconjugated (orb499747) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Sample: Lane 1: Recombinant human IL-6 protein, C-His, Lane 2: Recombinant human IL-6 protein, C-His, Primary: Anti-human IL-6 (orb499747) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 23 kDa, Observed band size: 15 kDa.

Quick Database Links

Gene Symbol

IL6