Interferon Gamma Antibody

• Catalog Number: orb345079
• Category: Antibodies
• Description: IFN gamma antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Bovine
• Isotype: IgG
• Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast corresponding to the 143 amino acids of the mature Bovine IFN Gamma protein.
• Antibody Type: Primary Antibody
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:20,000, WB: 1:500-1:2,000
• Form/Appearance: Lyophilized
• Purity: This product was protein A purified from monospecific antiserum by chromatography. This antibody is specific for bovine IFN gamma protein. A BLAST analysis was used to suggest cross-reactivity with IFN gamma from bovine based on 100% homology; cross-reactivity to yak, bison, zebu, buffalo, goat, sheep, nilgai, giraffe, Chinese forest musk deer, sika deer, red deer, Arabian camel, and Bactrian camel based on 91-99% homology with the immunizing sequence. Cross-reactivity with IFN gamma from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: A9QXB7
• NCBI: NP_776511.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-IFN gamma, rabbit anti-Interferon gamm
• Note: For research use only
• Application notes: This protein A purified IFN-gamma antibody has been tested by ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 16.9 kDa in size corresponding to bovine IFN gamma by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot using Biorbyt's protein-A purified anti-bovine IFN gamma antibody shows detection of recombinant bovine IFN gamma at 16.9 kDa, raised in yeast. Primary antibody was diluted to 1 µg/ml. 3% BSA from Biorbyt's Bovine Serum Albumin Solution was used for blocking. Secondary antibody (Goat anti-Rabbit IgG IRDye 800) was used at 1:20000.