IFN gamma Rabbit Polyclonal Antibody

SKU: orb501002

Description

IFN gamma Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, ICC, WB
• Dilution range: WB=1:500-2000, ICC/IF=1:50-200, Flow-Cyt=1:50-100
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human IFN gamma (1-100/166aa)
• Target: IFNG
• Molecular Weight: 15 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

IFNG; IFG; IFI; IFN gamma; IFN Immune; IFN-gamma; IFNG; IFNG_MOUSE; Immune Interferon; Interferon gamma; Interferon gamma Precursor; Macrophage Activating Factor; MAF; T Cell Interferon; Type II Interferon.

Blank control (black line): ctll-2. Primary Antibody (green line): Rabbit Anti-IFN gamma antibody (orb501002), dilution: 0.5 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

ctll-2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (IFN gamma) polyclonal Antibody, Unconjugated (orb501002) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (mouse lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IFN gamma) Polyclonal Antibody, Unconjugated (orb501002) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nuclei staining.

Paraformaldehyde-fixed, paraffin embedded (mouse lymph), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IFN gamma) Polyclonal Antibody, Unconjugated (orb501002) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nuclei staining.

Paraformaldehyde-fixed, paraffin embedded (rat lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IFN gamma) Polyclonal Antibody, Unconjugated (orb501002) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nuclei staining.

Paraformaldehyde-fixed, paraffin embedded (rat lymph), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IFN gamma) Polyclonal Antibody, Unconjugated (orb501002) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nuclei staining.

Sample: Disease Lung (Mouse) Lysate at 30 ug, Disease Spleen (Mouse) lysate at 30 ug, Primary: Anti-IFN gamma (orb501002) at 1/200 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/3000 dilution, Predicted band size: 15 kD, Observed band size: 18 kD.

Sample: Lane 1: Mouse Raw264.7-PMA Lysates, Lane 2: Mouse Lymph Lysates, Lane 3: Mouse Spleen Lysates, Lane 4: Mouse Thymus Lysates, Lane 5: Rat Lymph Lysates, Lane 6: Rat Thymus Lysates, Lane 7: Human Jurkat-TPA cell Lysates, Lane 8: Human Jurkat cell Lysates, Lane 9: Human HepG2 cell Lysates, Lane 10: Recombinant human IFN gamma Protein, Primary: Anti-IFN gamma (orb501002) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 15kDa, Observed band size: 25kDa.

Sample: Lane 1: Recombinant human IFN Gamma protein, N-His Tag, Primary: Anti-IFN gamma (orb501002) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 15 kDa, Observed band size: 16 kDa.

Tissue/Cell: Mouse lung tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-IFN gamma Polyclonal Antibody, Unconjugated (orb501002) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

IFNG

UniProt

P01579

References

Narges Maleki 1, Maryam Rezapour Kalkhorann 1, Mohammad Sajad Sajad Emami Aleagha 2, Amir Emami 3, Abdolamir Allameh MSCs engineered with secreted Klotho alleviate blood-brain barrier disruption and reduce neuroinflammation more effectively than MSCs in experimental autoimmune encephalomyelitis Stem Cell Res Ther, (2025)

PubMed: 40598580