Indirubin

SKU: orb1222214

Description

Indirubin is a potent and selective CDK inhibitor. Indirubin is the active ingredient of Danggui Longhui Wan, a mixture of plants that is used in traditional Chinese medicine to treat chronic diseases. Indirubin has been used to treat the symptoms of leukemia. Further biological explorations revealed the ability of indirubin to bind cyclin-dependent kinases and 6-bromoindirubin, extracted from mollusks, to bind glycogen synthase kinase-3. Studies have shown that Indirubin can help keep cancer cells from reproducing in rats. Indirubin also appears to reduce inflammation by inhibiting part of the immune response.(In Vitro):Indirubin (Couroupitine B) significantly inhibits Td-EC proliferation, migration, invasion, and angiogenesis.(In Vivo):Indirubin (Couroupitine B) (12.5 mg/kg, 25 mg/kg; intraperitoneal injected; once a day for 14 days) may reduce the pathological alterations in a dose-dependent manner.

Key Properties

• CAS Number: 479-41-4
• MW: 262.26
• Purity: >98% (HPLC)
• Formula: C₁₆H₁₀N₂O₂
• SMILES: C1=CC=C2C(=C1)/C(=C\3/C(=O)C4=CC=CC=C4N3)/C(=O)N2
• Target: GSK-3β , CDK2/CyclinA , CDK5/p35 , CDK1/CyclinB , C-Raf
• Solubility: DMSO : ≥ 41 mg/mL; 156.33 mM

Bioactivity

• In Vivo:
  Indirubin (Couroupitine B) (12.5 mg/kg, 25 mg/kg; intraperitoneal injected; once a day for 14 days) may reduce the pathological alterations in a dose-dependent manner. Animal model: Male mice (C57BL/6). Dosage: 12.5 mg/kg, 25 mg/kg. Administration: Indirubin (12.5 mg/kg, 25 mg/kg; intraperitoneal injected; once a day for 14 days). Result: Attenuated alterations of lung structure induced by BLM.
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• In Vitro:
  Indirubin (Couroupitine B) significantly inhibits Td-EC proliferation, migration, invasion, and angiogenesis. Cell Proliferation Assay Cell line: Human umbilical vein endothelial cells (HUVEC) line; tumor-derived endothelial cells (Td-EC). Concentration: 5, 10 μM. Incubation time: 24, 48, 72 hours. Result: Inhibited Td-EC proliferation in a dose- and time-dependent manner. Cell Invasion Assay Cell line: Human umbilical vein endothelial cells (HUVEC) line; tumor-derived endothelial cells (Td-EC). Concentration: 5 μM. Incubation time: overnight. Result: Inhibited Td-EC migration, invasion, and angiogenesis.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Indirubin | C.I. 73200, Couroupitine B, NSC 105327

Quick Database Links

Gene Symbol

GSK-3β , CDK2/CyclinA , CDK5/p35 , CDK1/CyclinB , C-Raf