IMPDH2 Antibody

• Catalog Number: orb622725
• Category: Antibodies
• Description: IMPDH2 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: JE40-87
• Tested applications: FC, ICC, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein within C-terminal human IMPDH2.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1:2,000 FC: 1:10-1:50 IP: 1:10-1:50 ICC/IF: 1:50-1:200 IHC: 1:50-1:200
• Form/Appearance: Liquid
• Purity: ProA affinity purified.
• Conjugation: Unconjugated
• MW: Calculated MW 56 kDa.
• UniProt ID: P12268
• Storage: Store at -20˚C.
• Buffer/Preservatives: 1*TBS (pH7.4), 1% rAlbumin, 40% Glycerol 0.05% Sodium Azide
• Alternative names: IMDH2_HUMAN antibody/ IMP (inosine monophosphate
• Background: Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of IMPDH2 on Daudi cell lysate using anti-IMPDH2 antibody at 1/2000 dilution

Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-IMPDH2 antibody. Counter stained with hematoxylin

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IMPDH2 antibody. Counter stained with hematoxylin

Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-IMPDH2 antibody. Counter stained with hematoxylin

Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-IMPDH2 antibody. Counter stained with hematoxylin

ICC staining IMPDH2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS

ICC staining IMPDH2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS

ICC staining IMPDH2 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS

Flow cytometric analysis of Daudi cells with IMPDH2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody