IL6/Interleukin 6 Antibody

• Catalog Number: orb1539004
• Category: Antibodies
• Description: IL6/Interleukin 6 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: E.coli derived recombinant Human IL-6.
• Dilution range: ELISA (0.5 - 2 µg/ml), IHC-P (5 µg/ml), WB (0.1 - 0.2 µg/ml)
• Conjugation: Unconjugated
• Target: IL6 / Interleukin 6
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Lyophilized from PBS, pH 7.2. Lyophilized from PBS, pH 7.2
• Alternative names: IL6, B-cell stimulatory factor 2, BSF-2, BSF2, CTL
• Note: For research use only
• Application notes: Resuspension: Reconstitute in sterile water to 0.1-1 mg/ml. . Further information: Immunohistochemistry: LS-B7482 in an antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IL-6 (1 ng/ml), a concentration of 0.20-0.40 ug/ml of this antibody is required. ELISA: To detect Human IL-6 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-6 (LS-C104546) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-6. Western Blot: To detect Human IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-6 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
• Expiration Date: 12 months from date of receipt.