IL1B Antibody

• Catalog Number: orb1261302
• Category: Antibodies
• Description: IL1B Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-269 of human IL1B (NP_000567.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 31kDa
• Target: IL1B
• UniProt ID: P01584
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: IL1B, interleukin 1, beta, IL-1, IL1-BETA, IL1F2,
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of mouse liver, using IL1B antibody (orb1261302) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of THP-1 cells using IL1B Polyclonal Antibody (orb1261302) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of mouse lung using IL1B Polyclonal Antibody (orb1261302) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.