IL-1 Beta Rabbit Polyclonal Antibody

SKU: orb10903

Description

IL-1 Beta Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:500-2000, IHC-F=1:500-2000, IF=1:500-2000
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human IL-1beta (101-200/269aa)
• Target: IL1B
• Molecular Weight: 17/32 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

IL1B_HUMAN; Interleukin-1 beta; interleukin 1 beta; IL1F2; IL-1 beta; Catabolin; IL-1; IL1F2; IL1beta; IL1-BETA; IL-1beta; IL-1 beta; IL-1β; IL1β; IL-1B; IL1B;

Blank control (black line): A431 (black) (The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with PBST for 30 min on room temperature), Primary Antibody (green line): Rabbit Anti-PTP1B antibody, Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test.

Sample: Lane 1: Normal Mouse Raw264.7 cell lysates, Lane 2: Raw264.7 treated with 100 ng/ml lipopolysaccharide (LPS) for 6h, then with 300 ng/ml Brefeldin A added after 3 hours, Primary: Anti-IL-1 Beta (orb10903) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 17/32 kDa, Observed band size: 35 kDa.

Tissue/Cell: Human cervical carcinoma tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-IL-1 Beta/IL-1B Polyclonal Antibody, Unconjugated (orb10903) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-IL-1 Beta/IL-1B Polyclonal Antibody, Unconjugated (orb10903) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

IL1B

UniProt

P01584

References

Xiangyi Kong 1, Can Ning 1, Zengenni Liang 2, Chenglin Yang 1, You Wu 1, Yuanyuan Li 1, Aoao Wu 1, Yongkang Wang 1, Siqi Wang 1, Hui Fan 1, Wenguang Xiao 1, Jing Wu 1, Zhiliang Sun 3, Zhihang Yuan Koumine inhibits IL-1β-induced chondrocyte inflammation and ameliorates extracellular matrix degradation in osteoarthritic cartilage through activation of PINK1/Parkin-mediated mitochondrial autophagy Biomed Pharmacother, 173, 116273 (2024)

PubMed: 38412715

Applications

WB

Reactivity

Rat

HH Mustafa, SMA Hassan, SA Muhammad Influence of platelet-rich plasma on RANKL and IL-1 immunohistochemical expression in periodontitis-related bone cell proliferation and differentiation The Saudi Dental Journal, (2024)

PubMed:

Applications

IHC

Reactivity

Rat