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IL10RA Antibody
Description
Images & Validation
−| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 22-230 of human IL10RA (NP_001549.2). |
| Target | IL10RA |
| Molecular Weight | Observed: 72kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human Interleukin 10 Receptor Alpha (IL10Ra) ELISA Kit [orb777848]
Human
31.25-2000 pg/mL
11.6 pg/mL
48 T, 96 TMouse Interleukin 10 Receptor Alpha (IL10Ra) ELISA Kit [orb778433]
Mouse
78.13-5000 pg/mL
27 pg/mL
48 T, 96 TRat Interleukin 10 Receptor Alpha (IL10Ra) ELISA Kit [orb780223]
Rat
0.16-10 ng/mL
0.061 ng/mL
96 T, 48 T

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Western blot analysis of extracts of various cell lines, using IL10RA antibody (orb1258435) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded rat brain using IL10RA Antibody (orb1258435) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using IL10RA Antibody (orb1258435) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using IL10RA Antibody (orb1258435) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using IL10RA antibody (orb1258435). Blue: DAPI for nuclear staining.
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IL10RA Antibody (orb1258435)
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