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| Catalog Number | orb1258435 |
|---|---|
| Category | Antibodies |
| Description | IL10RA Antibody |
| Target | IL10RA |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Form/Appearance | Liquid |
| Concentration | batch dependent |
| Buffer/Preservatives | Liquid |
| Purification | Affinity purification |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 22-230 of human IL10RA (NP_001549.2). |
| UniProt ID | Q13651 |
| MW | Observed: 72kDa |
| Tested applications | IF, IHC, WB |
| Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | IL10RA, CDW210A, HIL-10R, IL-10R1, IL10R, CD210, C Read more... |
| Research Area | Immunology & Inflammation |
| Note | For research use only |

Western blot analysis of extracts of various cell lines, using IL10RA antibody (orb1258435) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded rat brain using IL10RA Antibody (orb1258435) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using IL10RA Antibody (orb1258435) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using IL10RA Antibody (orb1258435) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using IL10RA antibody (orb1258435). Blue: DAPI for nuclear staining.
Mouse | |
78.13-5000 pg/mL | |
27 pg/mL |
Rat | |
0.16-10 ng/mL | |
0.061 ng/mL |
Human | |
31.25-2000 pg/mL | |
11.6 pg/mL |
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