IL10RA Antibody

• Catalog Number: orb1258435
• Category: Antibodies
• Description: IL10RA Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 22-230 of human IL10RA (NP_001549.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 72kDa
• Target: IL10RA
• UniProt ID: Q13651
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: IL10RA, CDW210A, HIL-10R, IL-10R1, IL10R, CD210, C
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using IL10RA antibody (orb1258435) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded rat brain using IL10RA Antibody (orb1258435) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using IL10RA Antibody (orb1258435) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using IL10RA Antibody (orb1258435) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using IL10RA antibody (orb1258435). Blue: DAPI for nuclear staining.