Anti-IL-10R [1B1.3a]

• Catalog Number: orb758899
• Category: Antibodies
• Description: Rat monoclonal antibody to IL-10R
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: 1B1.3a
• Tested applications: Blocking, FC
• Reactivity: Mouse
• Isotype: IgG1
• Immunogen: This antibody was raised by immunising rats with the purified recombinant extracellular region of mouse Interleukin 10 Receptor.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: IL-10R
• UniProt ID: Q61727
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD210; Interleukin-10 receptor subunit; Interleuki
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-IL-10R antibody 1B1.3a. RAW264.7 cells were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of 1B1.3a (orb758898, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed RAW264.7 cells with anti-IL-10R antibody 1B1.3a. Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 1B1.3a (orb758898) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758898, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.