Il1b Antibody

SKU: orb1272469

Description

Il1b Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, NeA, WB
• Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-1-beta (murine IL-1 beta).
• Target: Il1b
• Purification: Anti-mIL-1 beta specific antibody was purified by affinity chromatography employing immobilized mIL-1 beta matrix.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Il-1b, IL-1betaInterleukin-1 betaIL-1 beta

To detect mIL-1-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Murine IL-1-beta (orb1272468) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-1-beta.

To detect mIL-1-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

To detect mIL-1-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained colchicine injected mouse brain (polymorph layer of the dentate gyrus) tissue. The primary antibody was incubated at 0.5 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.

Quick Database Links

Gene Symbol

Il1b

UniProt

P10749

RefSeq:P10749