IL-1a Antibody

• Catalog Number: orb1272473
• Category: Antibodies
• Description: IL-1a Antibody
• Target: Il1a
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Mouse
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-1-alpha (murine IL-1 alpha).
• UniProt ID: P01582
• Tested applications: ELISA, NeA, WB
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Il-1aInterleukin-1 alphaIL-1 alpha
• Note: For research use only
• NCBI: P01582

To detect mIL-1-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Murine IL-1-alpha (orb1272472) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-1-alpha.

To detect mIL-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

To detect mIL-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained colchicine injected mouse brain (including the hippocampal fissure) tissue. The primary antibody was incubated at 1.0 mg/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.

This antibody stained colchicine injected mouse brain (including the hippocampal fissure) tissue. The primary antibody was incubated at 1.0 mg/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.