IL-1a Antibody

• Catalog Number: orb1272471
• Category: Antibodies
• Description: IL-1a Antibody
• Target: Il1a
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Rat
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant Rat IL-1alpha (Rat IL-1alpha).
• UniProt ID: P16598
• Tested applications: ELISA, NeA, WB
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IL-1 alphaInterleukin-1 alphaIL-1 alpha
• Note: For research use only
• NCBI: P16598

To detect Rat IL-1-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Rat IL-1-alpha (orb1272470) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-1-alpha.

To detect Rat IL-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-1-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

To detect Rat IL-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-1-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained colchicine injected rat brain (including the ventricles and the CA3 region of the hippocampus) tissue. The primary antibody was incubated at 1.0 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.

This antibody stained colchicine injected rat brain (including the ventricles and the CA3 region of the hippocampus) tissue. The primary antibody was incubated at 1.0 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.