IL12A , IL12B Antibody

SKU: orb1671478

Description

IL12A , IL12B Antibody

Research Area

Cancer Biology, Immunology & Inflammation, Infectious Disease & Virology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Produced in BALB/c x ICR F1 mice immunized with highly pure Recombinant Human IL-12. Anti-Human IL- 12-specific antibody was purified from ascites fluid by Protein A affinity chromatography.
• Target: IL12A , IL12B
• Purification: IL- 12-specific antibody was purified from ascites fluid by Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Interleukin-12, NKSF, CTL Maturation Factor (TCMF), Cytotoxic Lymphocyte Maturation Factor (CLMF), TSF p35: Interleukin-12 subunit alpha, IL-12 subunit p35, IL-12A, Cytotoxic Lymphocyte Maturation Factor 35 kDa subunit (CLMF p35), NK cell Stimulating Factor Chain 1 p40: Interleukin-12 subunit beta, IL-12 subunit p40, IL-12B, CytotoxicLymphocyte Maturation Factor 40 kDa subunit (CLMF p40), NK cell Stimulating Factor Chain 2

In a sandwich ELISA (assuming 100 μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 1000 pg/ml Recombinant Human IL-12 when used with Biotinylated Antigen Affinity Purified Anti-Human IL-12 as the detection antibody at a concentration of at least 0.25-0.5 μg/ml.

To detect Human IL-12 by Western Blot analysis this antibody can be used at a concentration of 0.50-1.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-12 is 0.50-1.0 ng/lane, under non-reducing conditions.

Quick Database Links

Gene Symbol

IL12A , IL12B

UniProt

P29459, P29460

RefSeq:p35, P29460, p40, P29459