Il12a , Il12b Antibody (Biotin)

• Catalog Number: orb1671445
• Category: Antibodies
• Description: Il12a , Il12b Antibody (Biotin)
• Target: Il12a , Il12b
• Clonality: Polyclonal
• Conjugation: Biotin
• Reactivity: Mouse
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Purification: IL-12 specific antibody was purified by affinity chromatography and then biotinylated
• Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mIL-12. Anti-Murine IL-12 specific antibody was purified by affinity chromatography and then biotinylated.
• UniProt ID: P43432, P43431
• Tested applications: ELISA, WB
• Application notes: Sandwich ELISA: To detect mIL-12 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyt’s Polyclonal Anti-Murine IL-12 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mIL-12. Western Blot To detect mIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-12 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Interleukin-12, NKSF, CTL Maturation Factor (TCMF)
• Research Area: Cancer Biology, Immunology & Inflammation, Infecti
• Note: For research use only
• NCBI: p40, p35, P43432, P43431

To detect mIL-12 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine IL-12 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mIL-12.

To detect mIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-12 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.