IL-12 Antibody (biotin)

• Catalog Number: orb1671445
• Category: Antibodies
• Description: IL-12 Antibody (biotin)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Mouse
• Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mIL-12. Anti-Murine IL-12 specific antibody was purified by affinity chromatography and then biotinylated.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Lyophilized
• Conjugation: Biotin
• Target: Il12a , Il12b
• UniProt ID: P43431, P43432
• NCBI: p40, P43431, P43432, p35
• Storage: Antibody is stable for at least 2 years from date of receipt at -20˚°C. The reconstituted antibody is stable for at least two weeks at 2-8˚°C. Frozen aliquots are stable for at least 6 months when stored at -20˚°C. Avoid repeated freeze-thaw cycles.
• Alternative names: Interleukin-12, NKSF, CTL Maturation Factor (TCMF)
• Note: For research use only
• Application notes: Sandwich ELISA: To detect mIL-12 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Murine IL-12 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mIL-12. Western Blot To detect mIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-12 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

To detect mIL-12 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine IL-12 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mIL-12.

To detect mIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-12 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.