IKKb Antibody

• Catalog Number: orb248108
• Category: Antibodies
• Description: NF-kB is sequestered in the cytoplasm by IkBa, unable to translocate to the nucleus. IkB is part of the canonical pathway for NF-kB activation. IKKb is part of the IkB kinase complex, along with a second catalytic subunit, IKKa, and a regulatory subunit, IKKg. Along with IKKa, IKKb phosphorylates IkBa leading to its degradation and release of inhibition of NF-kB. The RELA subunit of active NF-kB is then free to enter the nucleus and initiate a number of signaling cascades.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: IKB355
• Tested applications: WB
• Reactivity: Human, Mouse
• Isotype: Mouse IgG1, kappa
• Immunogen: A recombinant protein fragment of IKKb was used as the immunogen for this antibody.
• Dilution range: Western blot: 2-4ug/ml
• Purity: Protein G purified antibody
• Conjugation: Unconjugated
• Formula: 0.5 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This IKKb antibody is available for research use only.
• Entrez: 3551
• Storage: Aliquot the IKKb antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 1X PBS, pH 7.4
• Note: For research use only
• Application notes: Provided assay concentrations are suggestions only, IKKb antibody titration may be required for optimal results.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of human samples using IKKb antibody (primary antibody dilution at: at 2 ug/ml)