Anti-IKK beta/IKBKB Antibody

• Catalog Number: orb215975
• Category: Antibodies
• Description: Anti-IKK beta/IKBKB Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human IKK beta recombinant protein (Position: E398-S756). Human IKK beta shares 91% and 90% amino acid (aa) sequences identity with mouse and rat IKK beta, respectively.
• Antibody Type: Primary Antibody
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 43 kDa
• UniProt ID: O14920
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Inhibitor of nuclear factor kappa-B kinase subunit
• Note: For research use only
• Application notes: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
• Expiration Date: 12 months from date of receipt.

Anti-IKK beta antibody, Western blotting. All lanes: Anti IKK beta at 0.5 ug/ml. Lane 1: HEPG2 Whole Cell Lysate at 40 ug. Lane 2: COLO320 Whole Cell Lysate at 40 ug. Lane 3: M231 Whole Cell Lysate at 40 ug. Lane 4: HT1080 Whole Cell Lysate at 40 ug. Predicted bind size: 87 KD. Observed bind size: 87 KD.

Anti-IKK beta antibody, Western blotting. All lanes: Anti IKK beta at 0.5 ug/ml. WB: Recombinant Human IKK beta Protein 0.5 ng. Predicted bind size: 43 KD. Observed bind size: 43 KD.

Anti-IKK beta antibody, IHC(P): Human Lung Cancer Tissue.

Flow Cytometry analysis of 293T cells using anti-IKK beta antibody. Overlay histogram showing 293T cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IKK beta Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

IF analysis of IKK beta using anti-IKK beta antibody. IKK beta was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 µg/mL rabbit anti-IKK beta Antibody overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.