IgM Heavy Chain Antibody

• Catalog Number: orb248392
• Category: Antibodies
• Description: IgM is the first antibody generated in an immune response to an antigen. It is generally a pentamer with each of the five immunoglobulins linked together with disulfide bonds. In its pentamer form, it has a molecular mass of 970 kDa and 10 antigen binding sites (due to the large size of most antigens, not all binding sites can be filled simultaneously. IgM antibodies account for approximately 5%-10% of all the antibody in the body.This mAb is specific for the human IgM heavy chain. It does not cross-react with other immunoglobulin heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. IgM antibody is useful in the identification of certain cancer types. Some tumors express a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Purity: Protein G
• Immunogen: Recombinant human IgM was used as the immunogen for this anti-IgM antibody.
• Tested applications: IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: Due to differences in protocols and secondaries, the anti-IgM antibody may need to be titered for optimal performance.1. FFPE staining requires boiling sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: IM260
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Store the anti-IgM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Hazard Information: This anti-IgM antibody is available for research use only. It is not intended for use in diagnostic or therapeutic applications.
• Note: For research use only
• Entrez: 3507
• Expiration Date: 12 months from date of receipt.

IHC testing of human tonsil stained with anti-IgM antibody (IM260).

Western blot analysis of anti-IgM antibody and Raji cell lysate. Expected molecular weight: 70-75 kDa.

SDS-PAGE Analysis of Purified, BSA-Free Anti-IgM Antibody (clone IM260). Confirmation of Integrity and Purity of the Antibody.