Rabbit anti-IgM Recombinant Monoclonal Antibody

• Catalog Number: orb1806431
• Category: Antibodies
• Description: Rabbit anti-IgM Recombinant Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BLR294L
• Tested applications: FC, ICC, IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Human IgM
• Antibody Type: Primary Antibody
• Concentration: 100 µg/ml
• Dilution range: WB: 1:1000, IP: 20 µl/mg lysate, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: IgM
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human IgM by western blot. Samples: Whole cell lysate (10 µg) from Daudi, MOLT-4, Raji, HEK293T, and Ramos cells prepared using NETN lysis buffer. Antibody: Rabbit anti-IgM recombinant monoclonal antibody (orb1806431) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse IgM by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, WEHI-231, CT26, CH27, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-IgM recombinant monoclonal antibody (orb1806431) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human IgM by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-IgM recombinant monoclonal antibody (orb1806431). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human IgM by immunohistochemistry. Sample: FFPE section of B-cell lymphoma. Antibody: Rabbit anti-IgM recombinant monoclonal antibody (orb1806431). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human IgM (shaded) in Raji cells by flow cytometry. Antibody: Rabbit anti-IgM recombinant monoclonal antibody (orb1806431) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human IgM by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Ramos cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-IgM recombinant monoclonal antibody (orb1806431) used for IP at 20 µl/mg lysate.

Detection of human IgM by immunocytochemistry. Sample: FFPE section of Daudi cells. Antibody: Rabbit anti-IgM recombinant monoclonal antibody (orb1806431). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse IgM by immunocytochemistry. Sample: FFPE section of WEHI-231 cells. Antibody: Rabbit anti-IgM recombinant monoclonal antibody (orb1806431). Secondary: HRP-conjugated goat anti-rabbit IgG.