IGFBP3 Antibody

SKU: orb1246706

Description

IGFBP3 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 28kDa band observed in Human Breast Cancer lysates (calculated MW of 31.7kDa according to NP_001013416.1 and 32.2kDa according to NP_000589.2). Recommended concentration: 0.02-0.06µg/mL. An additional band of 50kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-RYKVDYESQSTDTQN
• Target: IGFBP3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IGFBP3, insulin-like growth factor binding protein 3, tcag7.703, BP-53, IBP3, IGF-binding protein 3, acid stable subunit of the 140 K IGF complex , binding protein 29, binding protein 53, growth hormone-dependent binding protein

Quick Database Links

Gene Symbol

IGFBP3

UniProt

P17936

RefSeq (Protein):NP_000589.2, NP_001013416.1