IGFBP3 Antibody

SKU: orb1272657

Description

IGFBP3 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits immunized with highly pure recombinant Rat SCF. Rat SCF specific antibody was purified by affinity chromatography employing an immobilized Rat SCF matrix.
• Target: IGFBP3
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IBP3, BP-53, IBP3, Insulin-like growth factor-binding protein 3IBP-3

To detect hIGF-BP3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IGF-BP3 (orb1272742) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP3.

To detect hIGF-BP3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect hIGF-BP3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained formalin-fixed, paraffin-embedded sections of normal human placenta. The recommended concentrations are 2.5 ug/ml-0.5 ug/ml for two hours at room temperature. An HRP-labeled polymer detection system was used with DAB chromogen. Heat induced antigen retrieval was performed with a pH 6.0 Sodium Citrate buffer. Optimal concentrations and conditions may vary.

This antibody stained formalin-fixed, paraffin-embedded sections of normal human placenta. The recommended concentrations are 2.5 ug/ml-0.5 ug/ml for two hours at room temperature. An HRP-labeled polymer detection system was used with DAB chromogen. Heat induced antigen retrieval was performed with a pH 6.0 Sodium Citrate buffer. Optimal concentrations and conditions may vary.

This antibody stained formalin-fixed, paraffin-embedded sections of normal human placenta. The recommended concentrations are 2.5 ug/ml-0.5 ug/ml for two hours at room temperature. An HRP-labeled polymer detection system was used with DAB chromogen. Heat induced antigen retrieval was performed with a pH 6.0 Sodium Citrate buffer. Optimal concentrations and conditions may vary.

Quick Database Links

Gene Symbol

IGFBP3

UniProt

P17936

RefSeq:P17936