IGF2R Antibody

• Catalog Number: orb1260633
• Category: Antibodies
• Description: IGF2R Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 2327-2491 of human IGF2R (NP_000867.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 310kDa
• Target: IGF2R
• UniProt ID: P11717
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: IGF2R, CD222 antigen, CIMPR, CI Man-6-P receptor,
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of HeLa cells, using IGF2R antibody (orb1260633) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human placenta using IGF2R antibody (orb1260633) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using IGF2R antibody (orb1260633) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using IGF2R antibody (orb1260633) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using IGF2R antibody (orb1260633) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using IGF2R antibody (orb1260633) at dilution of 1:100. Blue: DAPI for nuclear staining.